American Society of Hematology

Figure 7

$Figure 7. Radial localization and distances to closest nucleolus for IgH and c-myc loci in the Raji BL cell line. (A) Schematic representation of the chromosomes involved in the t(8;14) translocation in the Raji BL cell line and localization of the IgH and c-myc probes used. (B) Distribution of the translocated (IgH – c-myc der14) and nontranslocated (IgH 14 and c-myc 8) IgH and c-myc alleles according to their radial positioning in Raji cell nuclei. Distances were measured as described in Materials and methods. Histograms represent the percentage of FISH signals in the nuclear space with the range 0.0 to 0.15 corresponding to the centermost fraction of the nucleus and 0.75 to 1 to its periphery. Each graph represents a minimum of 115 FISH signals. (C) Perinucleolar relocalization of the der14 IgH and c-myc alleles in the BL Raji cell line. The IgH and c-myc alleles and nucleoli were revealed by immuno-FISH. Distances to the closest nucleolus were measured as described in Materials and methods for a minimum of 115 FISH signals. (D) A representative image of double-labeled DNA FISH in RAJI cell line. IgH is green, c-myc is red, and nucleolus (B23) is blue. Scale bar represents 5 µm.$

Radial localization and distances to closest nucleolus for IgH and c-myc loci in the Raji BL cell line. (A) Schematic representation of the chromosomes involved in the t(8;14) translocation in the Raji BL cell line and localization of the IgH and c-myc probes used. (B) Distribution of the translocated (IgH – c-myc der14) and nontranslocated (IgH 14 and c-myc 8) IgH and c-myc alleles according to their radial positioning in Raji cell nuclei. Distances were measured as described in Materials and methods. Histograms represent the percentage of FISH signals in the nuclear space with the range 0.0 to 0.15 corresponding to the centermost fraction of the nucleus and 0.75 to 1 to its periphery. Each graph represents a minimum of 115 FISH signals. (C) Perinucleolar relocalization of the der14 IgH and c-myc alleles in the BL Raji cell line. The IgH and c-myc alleles and nucleoli were revealed by immuno-FISH. Distances to the closest nucleolus were measured as described in Materials and methods for a minimum of 115 FISH signals. (D) A representative image of double-labeled DNA FISH in RAJI cell line. IgH is green, c-myc is red, and nucleolus (B23) is blue. Scale bar represents 5 µm.

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