Ccnd1 transcription levels and colocalization of nucleoli and Ccnd1. (A) A nucleolin-GFP pEGFPC1. Nucelolin construct was transfected into HeLa cells followed by selection of GFP-positive cells by flow cytofluorimetry. Transcription of nucleolin, Ccnd1, c-myc, Bcl2, Actin B, and 18S rRNA studied by quantitative reverse-transcription PCR in GFP-nucleolin and mock-transfected cells. Error bars represent the standard deviation between duplicates. (B-C) Ccnd1 localization and expression in Granta-519 cells treated with a histone deacetylase inhibitor. The localization of a Ccnd1 allele <1 µm from a nucleolus (B) as determined using 3D-FISH and Ccnd1 transcription rate (C) as measured by quantitative reverse-transcription PCR. The data represent the average results from 2 independent experiments.