Serial mutational analysis of genes outside of JAK2 during therapy with PEG-IFN-α-2a in patients with PV and ET. Graphical representation of serial mutational analysis in patients based on JAK2V617F allele burden response. (A) Somatic mutations in granulocyte DNA that were acquired (red boxes), retained (orange boxes), or lost (purple boxes) in follow-up samples compared with pretreatment samples. Also displayed are the JAK2V617F mutant allele burden values (determined by JAK2V617F ASP) in peripheral blood granulocyte DNA at the time in which extended mutational profiling was performed. (B) Sanger sequencing electropherograms displaying acquisition and/or loss of mutations from individual patients during the course of PEG-IFN-α-2a therapy.