Tregsdown-regulate CD86 and CD54 on DCs. Flow analysis on DCs from skin-draining lymph nodes (sLNs) and spleens of recipient mice that were transplanted with T cells or eTregs or iTregs (with TCD BM) 1 day or 2 days prior to analysis. Ctrl: antibody isotype control. No treatment: mice were not irradiated or transplanted prior to evaluation. BM: mice were transplanted with BM only. W/ T: mice transplanted with donor T cells. W/ e Treg: mice transplanted with endogenous Treg. W/ iTregs: mice transplanted with iTregs. (A) The histogram of CD86 and CD54 expression on DCs from spleens of mice transplanted with T cells or eTregs 2 days posttransplant. (B) The fold change of mean fluorescence intensity (MFI) for CD86 and CD54 on DCs was calculated by dividing the MFI of the specific staining antibody by the MFI of the control antibody. N = 3 to 4 mice per group (1 mouse for no treatment) with representative data from 2 different experiments. (C) The histogram of CD86 and CD54 expression on DCs from sLNs of mice transplanted with T cells or iTregs 1 day posttransplant. (D) MFI of CD86 and CD54 on DCs from (C). N = 3 to 4 mice per group (1 mouse for no treatment) with representative data from 2 different experiments. No difference was found in the expression of CD80, CD40, MHCII, B7H3, B7H4, or CD70 on DCs in the presence or absence of eTregs or iTregs (data not shown).
