USP10 in T cells controls the SG-forming activity and sensitivity to arsenite-induced apoptosis. (A) USP10-knockdown Jurkat cells and control cells were treated with 0.25 mM of sodium arsenite for 0, 20, and 30 minutes and then were stained with anti-G3BP1 antibodies (red) and Hoechst33258 (blue). The bars indicate 10 μm. (B) The SG (%) is presented. (C) USP10-knockdown Jurkat cells and control cells were treated with 5 μM of sodium arsenite for 48 hours and then were stained with propidium iodide (PI). The proportion of the sub-G1 fraction was measured using flow cytometry. In all experiments, the values denote the mean ± SD; *P < .05; **P < .01; ***P < .001.