Effect of latrunculin B, blebbistatin, aprotinin, TXA, EACA, and GM-6001 on polarization of transmigrated leukocytes. (A) Representative in vivo transillumination microscopy images of transmigrated leukocytes in the cremasteric interstitial tissue of animals treated with latrunculin B, blebbistatin, aprotinin, TXA, EACA, GM-6001, or vehicle. As a measure of leukocyte polarization, eccentricity of transmigrated leukocytes was determined, as detailed in the “Methods” section. Panels show results for mice receiving (B) latrunculin B, blebbistatin, or drug vehicle, (C) aprotinin, TXA, EACA, or drug vehicle, and (D) GM-6001 or drug vehicle (mean ± SEM for n = 4 per group; *P < .05 vs vehicle).