Tumor-suppressive effect of miR-150 in T-cell lymphomas. (A) qPCR analysis of miR-150 expression in the indicated cell types transduced with GFP-empty (control) (red bars) vector or GFP-miR-150 (green bars). GFP-control or GFP-miRNA transfected cells were sorted and GFP-positive cells with >99% purification was obtained. X-axis: GFP-control or GFP-miR-150–transfected cell lines (ATN-1, MJ, HUT78, My-La, and HH). Y-axis: red and green bars depict ^−ΔΔCt values for miR-150 expression in the 5 transfected cell lines. Bars are means ± SEM of 3 independent experiments. (B) Northern blot analysis of the indicated miR-150 in indicated cells transfected with GFP-miR-150 (shown as “miR-150”) or empty control vector (shown as “control”). The transfected cell type is indicated at the top. Controls (5S tRNA) are shown in the bottom panel. (C) NOG mouse survival after engraftment of GFP-miR-150–transfected My-La or HH cells. Two independent inoculation assays are shown [My-La or HH-GFP-control(1), My-La or HH-GFP-control(2), My-La or HH-miR-150(1), My-La or HH-miR-150(2); n = 5 each]. X-axis: days from inoculation (day 0); Y-axis: survival rate. Significance (P) was determined using the Kaplan-Meier method.