Immunohistochemical evidence for mural cell recruitment after PlGF treatment. Immunostaining of endothelial cells for (A,C,E,G,I,) podocalyxin (green) and for (B,D,F,H,J) pericytes (PDGFR-β, red). (K-L) Quantitative analysis of (K) PDGFR-β–positive area, and quantitative comparison of (L) podocalyxin vs PDGFR-β–positive areas, with horizontal line at 1, referring to equal area with both stainings. (E-H) Arrowheads point to hemangiomas. ***P < .001 when compared with HSA control; error bars show SD. Scale bar, 50 μm.