Effect of SCD on stroke after MCA occlusion. At 1 hour following MCA occlusion, the area of erythema representing ischemic zone was greater in SCD compared with WT mice (A-C). At 3 days following MCA occlusion, infarct size by 2,3,5-triphenyltetrazolium chloride staining was larger in SCD mice compared with WT BM mice (D-F). Microvascular occlusions by sickled RBCs were widespread in brain sections of SCD BM mice (G), but not in WT mice. Bar = 50 μm. *P < .05. BM, bone marrow.