Selective ERβ agonist inhibits growth and dissemination of Raji BL cells in vivo. (A) Expression of ERα and ERβ mRNA in Raji BL was measured by quantitative real-time PCR. (B) Male NOD-SCID mice were subcutaneously grafted with 5 × 106 Raji BL cells. Starting from day 12 after tumor cell grafting, animals were treated subcutaneously daily with the ERβ-selective agonist DPN (12.5 μmol/kg per day) (▪) or vehicle (●). The number (C) and size (D-E) of tumor foci of Raji cell dissemination in liver were significantly reduced in DPN-treated mice with Raji lymphomas as evaluated using hematoxylin and eosin staining. Original magnification ×200. The groups consisted of 4 (vehicle-treated) and 6 (DPN-treated) mice. For vehicle vs DPN: *P < .05, **P < .01, and ***P < .001. Data are representative of at least 2 independent experiments.
