Comparative imaging of CX3CR1^gfp/+ and MacBlue mice. (A) Representative 2-photon laser scanning microscopy (TPLSM) images of mouse skull bone tissues from CX3CR1^gfp/+ and MacBlue mice. GFP⁺ cells appear in green, CFP⁺ cells appear in cyan, and the microvasculature was visualized using rhodamine-dextran (red). The bone matrix is visualized by second harmonic generation (SHG; blue). (B) Representative dot plots of GFP and CFP coexpression and CD11b, Ly6C, and Ly6G expression gated on the indicated populations from MacBlue-CX3CR1^gfp/+ mouse (mean percentage ± SD from n = 6 different mice pooled from 2 independent experiments are indicated). (C) Impact of CP treatment on GFP and CFP expression on skull bone marrow total cell from CX3CR1^gfp/+ and MacBlue mice.