Increased donor T_con accumulation within host tissues in recipients of CCR8 ^−/− T_regs. (A-B) B6D2 recipients were lethally irradiated on day −1 and then administered 3 × 10⁶ TCD BM cells from WT B6 donors plus 1 × 10⁶ column-purified CD4⁺CD25⁺ T_regs from WT eGFP^– B6 or CCR8^−/− eGFP^– B6 mice on day 0. Then 4 × 10⁶ CD25-depleted eGFP⁺ T_cons from WT B6 donors were administered on day +2. Recipient animals were anesthetized on transplant days +14 (A) and day +21 (B), and their organs imaged by fluorescence stereomicroscopy. Total eGFP levels were then quantified in these sites using an eGFP ELISA kit. (A) n = 4 recipients per group. (B) n = 5 recipients per group. *P = .032 for total eGFP comparison between WT and CCR8^−/− T_reg recipients by the Mann-Whitney test. **P = .016.