Decreased accumulation of CCR8 ^−/− T_regs after transplant day +7. (A-C). B6D2 recipients were lethally irradiated on day –1 and then administered 3 × 10⁶ TCD BM cells from WT eGFP^– B6 donors plus 1 × 10⁶ column-purified CD4⁺CD25⁺ T_regs from WT eGFP⁺ B6 or CCR8^−/− eGFP⁺ B6 mice on day 0. Then 4 × 10⁶ CD25-depleted eGFP^– T_cons from WT B6 donors were administered on day +2. Recipient animals were anesthetized on transplant days +7 (A), +10 (B), or +14 (C), and lymphoid organs imaged by fluorescence stereomicroscopy. Total eGFP levels were then quantified in these sites using an eGFP ELISA kit. (A) n = 4 recipients per group. (B) n = 5 recipients per group. *P = .008 for total eGFP comparison between WT and CCR8^−/− T_reg recipients by the Mann-Whitney test. **P = .032. (C) n = 8 WT T_reg and 5 CCR8^−/− T_reg recipients. *P = .019; **P = .011.