siRNA-mediated depletion of MAPKAPK3 and KIT signaling molecules downregulates PAX5 expression in Kasumi-1 cells. (A-B) Immunostaining showing KIT and MAPKAPK3 proteins (red), respectively, in Kasumi-1 cells after siRNA-mediated depletion. (siNEG) cells transfected with a control siRNA. (C) qRT-PCR showing expression of PAX5, RPL13A, and TBP relative to GAPDH in Kasumi-1 cells transfected with control siRNA (siNEG), MAPKAPK3 siRNA, and KIT siRNA. Values obtained with siNEG were set as one. The bar graph is representative of 2 independent experiments measured in duplicate; the error bars show variability in qPCR measurements.