PAX5 is neither a direct nor an indirect target of RUNX1/ETO. (A) qRT-PCR showing (A,C) RUNX1/ETO and (B,D) PAX5 expression after transfecting (left) a primary t(8;21) sample and (right) Kasumi-1 cell line with siRNA mismatch (siRNA MM) and siRNA RUNX1/ETO (siRNA RE) to knock-down RUNX/ETO. Materials were from a previous study.⁵  qRT-PCR, quantitative reverse transcription PCR.