PAX5 is poised for transcription in non-t(8;21) myeloid precursors and is repressed by polycomb-repressive complexes. ChIP-qPCR experiments measuring relative enrichment of (A) H3K4me3, (B) H3K9ac, (C) total RNA Pol II, (D) elongating form of RNA Pol II, (E) H3K27me3, (F) H3K27ac, and (G) EZH2 at the PAX5 promoter hypersensitive sites. Relative enrichment is calculated over the inactive IVL locus in panels A, B, C, and D and relative to input in E and F and relative to TBP in panel G. Each bar graph is representative of at least 2 independent experiments analyzed in duplicate. The error bars represent variability in qPCR measurements.