Plzf depletion prolongs MLL-ENL–induced leukemia. (A) Experimental strategy for the leukemia model using secondary BMT of primary MLL-ENL–leukemic cells. (B-C) Expression levels of Plzf by QRT-PCR (B) and relative CFU (C) of the cells sorted from shRNA-transduced leukemic cells. (D) Survival curves of the mice secondarily transplanted with the same Plzf-depleted (ME+shP01; n = 16) or control (ME+shLuc; n = 12) leukemic cells that are analyzed in panels B,C. Data from 3 independent experiments using independent primary leukemic cells were combined. (E-F) Expression levels of Plzf by QRT-PCR (E) and typical immunophenotype (F) of splenocytes from disease mice. The bar graphs show the mean ± SD of 3 independent experiments.