Glycol-split heparins reduce basal and BMP6-induced hepcidin in HepG2 in 4 to 6 hours. (A) HepG2 cells were treated with 3.6 μg/mL glycol-split heparins RO-82 and RO-68 at different times (30 minutes-1 hour-2 hour-4 hour-6 hour-16 hour) without BMP6. The values are expressed as percentage of untreated cells (100%). (B) In presence of BMP6 (10 ng/mL). Hepcidin mRNA levels were quantified with qRT-PCR in relationship to Hprt1 mRNA. The values are expressed as fold increase of untreated cells. Data means and SD of 3 different experiments.