Effects of dasatinib on various myeloid cell subsets. (A) CD14⁺ monocytes, CD1c⁺ myeloid DCs, and slan⁺ DCs were purified from peripheral blood mononuclear cells. After overnight culture, the cells were stimulated with LPS/IFNγ with or without 50 nM of dasatinib and were tested for cytokine production after 16 hours. (B) Dasatinib sensitizes different cells of myeloid origin for suboptimal LPS triggering. CD14⁺ monocytes were subjected to maturation with titrated amounts of LPS in the absence or presence of dasatinib (50 nM). IL-12p40 production was detected after 16 hours of maturation. Results are representative for at least 3 experiments.