Effects of dasatinib on DC function. (A) Effect of dasatinib on IL-12 production. DCs were matured with various maturation cocktails alone (upper row) or in the presence of dasatinib (lower panel). Intracellular IL-12p40 and IL-8 were stained after 16 hours of stimulation. (B) Analysis of 10 different donors for production of IL-12p40 assessed by intracellular cytokine staining. DCs were matured with the respective stimuli and were coincubated with dasatinib (50 nM, red bars) for 16 hours. Indicated is the mean percentage of IL-12p40+ DCs and the standard deviation. (C) Dose titration of dasatinib on maturing DCs. DCs were matured with LPS/IFNγ in combination with the indicated amounts of dasatinib (1 of 2 experiments with comparable results). (D) Effects of other tyrosine kinase inhibitors on 10 different donors. DCs were matured with LPS/IFNγ and were coincubated with 50 nM of dasatinib, 10 µM of imatinib, or 1 µM of nilotinib for 16 hours, followed by intracellular cytokine staining for IL-12p40 and TNFα.