WHIM-mutant CXCR4-mediated impairment of T-DC interactions in ex vivo lymph node slice cultures is reversed by the CXCR4 inhibitor AMD3100. DCs were either cognate antigen-pulsed (OVA) or unpulsed (no pept). (A) Duration of T-DC interactions within ex vivo lymph node slices imaged by 2-photon microscopy in the presence of 12.6 μM AMD3100. The summary results of 2 videos per condition are shown. Each dot represents a single T-DC interaction (from left to right, n = 150, 103, 167, 158, 152, 301, 137, and 178). A Fisher’s exact test was applied to the number of interactions ≥20 minutes versus the number of interactions <20 minutes. *P < .05; ***P < .001. (B) The percentage of T-DC interactions ≥20 minutes, out of all analyzed interactions. White bars indicate nonretrogenic OVA-specific control T cells; gray bars indicate OVA-specific T cells retrogenically expressing WT CXCR4; black bars indicate OVA-specific T cells retrogenically expressing WHIM-mutant CXCR4. The statistical analysis refers to the Fisher’s exact test, as in (A). ctrl, nonretrogenic control OVA-specific T cells; WT, OVA-specific T cells retrogenically expressing WT CXCR4; WHIM, OVA-specific T cells retrogenically expressing WHIM-mutant CXCR4. See also supplemental Video 6. n.s., not significant.