DJ-1 binds to mortalin in the mitochondria of HSCs under conditions of oxidative stress. (A) Lin⁻ EML cell lysates were immunoprecipitated with mortalin- or DJ-1–specific antibodies, and the bound complexes were subjected to western blot analysis for mortalin and DJ-1. (B) qRT-PCR analysis of DJ-1 in CD150⁺CD48⁻ LSK, CD48⁺ LSK, Lin⁻, and Lin⁺ cell populations. β-actin was used as an endogenous control. Data represent the mean ± SD (n = 4; *P < .05, ***P < .001). (C) LSK cells were stained with mortalin Ab (green), DJ-1 pAb (red), and DAPI (blue), either with or without H₂O₂ (300 μM) treatment, for 2 hours.