Expression of developmental markers on NK cells from multiple organs. NK cells were identified as FSC-H versus FSC-A singlet and viable cells and then as NKp46⁺CD3⁻ cells. Positive gates were set using fluorescence minus 1 with isotype controls. Circles represent control Npc1^+/+ mice and squares represent affected Npc1^−/− mice; ages are ±1 day. Data are presented as mean ± SEM, n = 3 to 7 animals. *P < .05, **P < .01, and ***P < .001, calculated by an unpaired t test comparing to the age-matched controls, using GraphPad Prism v4.