Figure 1
Figure 1. NK-cell percentage and number in multiple organs from control and NPC1 animals at end stage. (A) NK cells as a percentage of CD45+ live cells and (B) NK-cell number per spleen or per 2 inguinal lymph nodes. Data were gated on forward scatter height (FSC-H) vs forward scatter area (FSC-A) singlets and dead cells were excluded. CD45+ cells were then selected and NK cells defined at NKp46+CD3− cells within that gate. Clear bars represent control Npc1+/+ animals and solid bars represent affected Npc1−/− animals. Data are presented as mean ± standard error of the mean (SEM), n = 3 to 10 animals with 2 independent determinations of NK-cell frequency per animal, or n = 5 for NK-cell number. *P < .05, **P < .01, and ***P < .001, calculated with the Mann-Whitney U test using GraphPad Prism v4.

NK-cell percentage and number in multiple organs from control and NPC1 animals at end stage. (A) NK cells as a percentage of CD45+ live cells and (B) NK-cell number per spleen or per 2 inguinal lymph nodes. Data were gated on forward scatter height (FSC-H) vs forward scatter area (FSC-A) singlets and dead cells were excluded. CD45+ cells were then selected and NK cells defined at NKp46+CD3− cells within that gate. Clear bars represent control Npc1+/+ animals and solid bars represent affected Npc1−/− animals. Data are presented as mean ± standard error of the mean (SEM), n = 3 to 10 animals with 2 independent determinations of NK-cell frequency per animal, or n = 5 for NK-cell number. *P < .05, **P < .01, and ***P < .001, calculated with the Mann-Whitney U test using GraphPad Prism v4.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal