American Society of Hematology

Figure 1

$Figure 1. Inhibition of MYD88 is associated with decreased survival, while overexpression of MYD88 L265P promotes survival of WM cells. (A) The efficiency of MYD88 knockdown by 2 lentiviral shRNAs in BCWM.1 cells was confirmed by western blot using GAPDH as a loading control. (B) Relative survival of MYD88 L265P-expressing WM cells, BCWM.1, following knockdown of MYD88 by 2 lentiviral shRNAs targeting the 3′ UTRs of MYD88. P values are labeled compared with control shRNA. (C) Time-dependent survival of MYD88 L265P-bearing BCWM.1 and MWCL-1 cells, and MYD88 WT-expressing MEC-1, Ramos, and U266 cells following knockdown of MYD88. Cells were sorted by GFP for shRNA#1 expression at day 4 after lentiviral transduction. The percentage of GFP+, shRNA#1-expressing cells relative to the GFP−, shRNA#1-negative fraction at the indicated days after sorting is plotted and normalized to day 0 (right after sorting) values. (D) Relative survival of BCWM.1 and MWCL-1 WM cells engineered to overexpress WT or L265P MYD88 coding regions, with concurrent knockdown of endogenous MYD88 expression by 3′ UTR targeting shRNA#1. All experiments were performed at least in triplicate; P values are shown as labeled.$

Inhibition of MYD88 is associated with decreased survival, while overexpression of MYD88 L265P promotes survival of WM cells. (A) The efficiency of MYD88 knockdown by 2 lentiviral shRNAs in BCWM.1 cells was confirmed by western blot using GAPDH as a loading control. (B) Relative survival of MYD88 L265P-expressing WM cells, BCWM.1, following knockdown of MYD88 by 2 lentiviral shRNAs targeting the 3′ UTRs of MYD88. P values are labeled compared with control shRNA. (C) Time-dependent survival of MYD88 L265P-bearing BCWM.1 and MWCL-1 cells, and MYD88 WT-expressing MEC-1, Ramos, and U266 cells following knockdown of MYD88. Cells were sorted by GFP for shRNA#1 expression at day 4 after lentiviral transduction. The percentage of GFP⁺, shRNA#1-expressing cells relative to the GFP⁻, shRNA#1-negative fraction at the indicated days after sorting is plotted and normalized to day 0 (right after sorting) values. (D) Relative survival of BCWM.1 and MWCL-1 WM cells engineered to overexpress WT or L265P MYD88 coding regions, with concurrent knockdown of endogenous MYD88 expression by 3′ UTR targeting shRNA#1. All experiments were performed at least in triplicate; P values are shown as labeled.

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