Augmented vascular inflammation in Fpr2/3^−/−mice as assessed by intravital microscopy. Wild-type (Fpr2/3^+/+) and null (Fpr2/3^−/−) mice were subjected to 30 minutes’ clamping of the superior mesenteric artery, followed by a reperfusion phase lasting 45 to 180 minutes. Postcapillary venules were imaged and recorded for offline quantitation of white blood cell interaction with the endothelium. In parallel experiments, tissue was excised and analyzed by confocal microscopy. (A) Number of cell adhesion and (B) emigration in the postcapillary venules, as measured at different times after reperfusion. Representative light microscopy images are shown for sham (C-D) and 90-minute post-reperfusion (E-F) vessels. (G-H) Confocal images for postcapillary venules from Fpr2/3^+/+ and Fpr2/3^−/− mice, respectively, after 90 minutes of IR protocol. Green, vessel vascular endothelium staining; blue, neutrophil MRP14 marker. Data are mean ± SEM of 6 to 8 mice per group. *P < .05, **P < .01 vs respective Fpr2/3^+/+ value.