ASB2α is dispensable for the generation of BMDCs. (A) Experimental outline of ASB2 deletion and generation of ASB2α^−/− BMDCs. (B) PCR products from mouse BM DNA of 2 Mx1-Cre;ASB2^+/+ (^+/+) and 2 Mx1-Cre;ASB2^fl/fl (^−/−) mice after poly(I·C) administration using primers A and C, allowing the amplification of the knockout allele (Figure 3). (C) Relative expression of ASB2α mRNAs in BM cells of ASB2^+/+ and ASB2^−/−. (Sample size: ^+/+ = 22; ^−/− = 24). (D) Expression of CD11c, CD86, and MHC-II at the cell surface of ASB2α^−/− and ASB2α^+/+ BMDCs. (Sample size: ^+/+ = 16 and ^−/− = 16 for CD11c; ^+/+ = 14 and ^−/− = 14 for CD86; ^+/+ = 7 and ^−/− = 7 for MHC-II). (E) Relative expression of ASB2α mRNAs in BMDCs generated from BM cells of ASB2^+/+ and ASB2^−/−. (Sample size: ^+/+ = 22; ^−/− = 25). ***P < .001.