Figure 1.
Figure 1. Morphological and histological abnormalities in Clec1b-deficient lung. (A) Neonatal lethality of Clec1b+/+ and Clec1b−/− mice. Numbers within parentheses: dead/total neonates at P0. *P = .0000, Fisher’s exact test. (B) Cyanotic appearance of whole-body and lung air inflation failure in Clec1b−/− neonates. (C) Ventral views of E16.5/E17.5/E18.5 fetal lungs; magnified views of framed areas in images are shown on the right. (D) Hematoxylin and eosin staining of E16.5/E17.5/E18.5 lung sections. (E-F) Quantification of alveolar area (E) and alveolar septum thickness (F); mean ± SD, n = 3 each. *P = .0023; **P = .0003; ***P= .0414, Student t test. (G) Elastica van Gieson staining of P0 lung sections. Magnified views of framed areas in images are shown on the right. Black and open arrowheads: mature elastic fibers (dark purple) and alveolar septa without elastic fibers, respectively. Scale bars: 1 mm (B-C); 25 μm (D); 10 μm (G).

Morphological and histological abnormalities in Clec1b-deficient lung. (A) Neonatal lethality of Clec1b+/+ and Clec1b−/− mice. Numbers within parentheses: dead/total neonates at P0. *P = .0000, Fisher’s exact test. (B) Cyanotic appearance of whole-body and lung air inflation failure in Clec1b−/− neonates. (C) Ventral views of E16.5/E17.5/E18.5 fetal lungs; magnified views of framed areas in images are shown on the right. (D) Hematoxylin and eosin staining of E16.5/E17.5/E18.5 lung sections. (E-F) Quantification of alveolar area (E) and alveolar septum thickness (F); mean ± SD, n = 3 each. *P = .0023; **P = .0003; ***P= .0414, Student t test. (G) Elastica van Gieson staining of P0 lung sections. Magnified views of framed areas in images are shown on the right. Black and open arrowheads: mature elastic fibers (dark purple) and alveolar septa without elastic fibers, respectively. Scale bars: 1 mm (B-C); 25 μm (D); 10 μm (G).

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