Figure 5
Effect of sorafenib, PI3K, Raf, and MEK inhibitors on the viability and signaling pathways in primary human CLL cells. (A) CLL B cells purified from freshly isolated or freeze-thawed PBMCs from CLL patient samples were treated with 0.1 to 5 µM of the PI3K inhibitor AS605240 for 24 hours, and cell survival was determined by flow cytometry (mean ± SEM, n = 4). (B) CLL B cells were treated with a single dose of 10 µM sorafenib, Plexxikon 4720 (PLX), or the Mek-Inhibitor U01206 for 24 hours, and cell survival was determined by flow cytometry (mean ± SEM, n = 10). (C) CLL B cells were treated with a single dose of 10 µM sorafenib, PLX, or U01206 and immunoblotted for Erk phosphorylation and expression. One representative result from 4 independent experiments is shown. (D) Healthy B cells or CLL B cells purified from freshly isolated or freeze-thawed PBMCs from CLL patient samples were lysed and immunoblotted for Ras expression. Four representative samples from 8 donor-derived lysates from each group are shown. n.s., not statistically significant.

Effect of sorafenib, PI3K, Raf, and MEK inhibitors on the viability and signaling pathways in primary human CLL cells. (A) CLL B cells purified from freshly isolated or freeze-thawed PBMCs from CLL patient samples were treated with 0.1 to 5 µM of the PI3K inhibitor AS605240 for 24 hours, and cell survival was determined by flow cytometry (mean ± SEM, n = 4). (B) CLL B cells were treated with a single dose of 10 µM sorafenib, Plexxikon 4720 (PLX), or the Mek-Inhibitor U01206 for 24 hours, and cell survival was determined by flow cytometry (mean ± SEM, n = 10). (C) CLL B cells were treated with a single dose of 10 µM sorafenib, PLX, or U01206 and immunoblotted for Erk phosphorylation and expression. One representative result from 4 independent experiments is shown. (D) Healthy B cells or CLL B cells purified from freshly isolated or freeze-thawed PBMCs from CLL patient samples were lysed and immunoblotted for Ras expression. Four representative samples from 8 donor-derived lysates from each group are shown. n.s., not statistically significant.

Close Modal

or Create an Account

Close Modal
Close Modal