Microthrombocytopenia in ADAP-deficient mice. (A) Platelet count per microliter and (B) MPV were determined from Adap^−/−, Adap^+/− and control mice by a hematology analyzer. (A-B) Values are mean ± standard deviation (SD; n = at least 8). ***P < .001; *P < .05. (C) Transmission electron micrographs from Adap^+/+ and Adap^−/− platelets; scale bars represent 2 µm (top panel) and 1 µm (bottom panel). (D) ADAP localization in resting control platelets was assessed by confocal microcopy; scale bar represents 2 µm. Line plot histogram of the marked platelet in the bottom right corner. (E) Images of the platelet cytoskeleton ultrastructure on poly-l-lysine. Scale bars represent 1 µm (top panel) and 0.5 µm (bottom panel; i, ii).