Figure 2
![Figure 2. TEL-AML1 induces phosphorylation of STAT3. (A) Western blot analysis of p-S727 and p-Y705 STAT3 and total STAT3 in mouse c-kit+Ter119- fetal liver HPC, 72 hours after transduction with murine stem cell virus TEL-AML1 expressing (T/A) or empty (MSCV CD2) retroviral vectors. Numbers represent densitometric quantitation of phospho-STAT3 bands normalized to total STAT3. (B) Flow cytometric analysis of p-Y705 STAT3 in Reh 6 days after transduction with control scramble (mean fluorescence intensity [MFI] = 21.04) or shTA shRNA (MFI = 12.01) shRNA. Isotype control staining (MFI = 10.23) is also shown. All data are representative of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/122/4/10.1182_blood-2012-11-465252/4/542f2.jpeg?Expires=1724251771&Signature=WUGLUQxtXjqVBR5Z8-UIXejk3i-CVIdEy9rOtPc4K--cW6hGUBNQoalFcY0qiB3Gx6J7lb7Yta1BvBNwiB87qcit33qcqecbqBY3PLmJ0FdeSAmMplrmyQx-QjueLNYzjEecq1CJ1GgYFPkNM6M1JH4Ew9rmWp11PNf5TCZXMBR-n7x9LBADoj3lFm9NopCEuADnqNf3B4MuuKzHkt94CTk0OTjm4dMSiID0GjbYPcjXv3LO9z1VZqgf-h35D7T7Uw6-BquMJkZdyL03BeED3vbTFPy2GtD2EOMQLRe0Opzd-NHPyxsmj-4RrFgTlIrsNPUnf36ZkUmSEwnx9dz01Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TEL-AML1 induces phosphorylation of STAT3. (A) Western blot analysis of p-S727 and p-Y705 STAT3 and total STAT3 in mouse c-kit+Ter119- fetal liver HPC, 72 hours after transduction with murine stem cell virus TEL-AML1 expressing (T/A) or empty (MSCV CD2) retroviral vectors. Numbers represent densitometric quantitation of phospho-STAT3 bands normalized to total STAT3. (B) Flow cytometric analysis of p-Y705 STAT3 in Reh 6 days after transduction with control scramble (mean fluorescence intensity [MFI] = 21.04) or shTA shRNA (MFI = 12.01) shRNA. Isotype control staining (MFI = 10.23) is also shown. All data are representative of 3 independent experiments.
