Antigen-specific DC vaccination enhanced #213 scFv CAR-T cells accumulation in periphery and activation in tumor tissues. Three-day K562-A24 bearing NOG mice were transferred with #213 scFv CAR-T cells or CEA-specific (F11-39 scFv) CAR-T cells (1 × 10⁷ cells) followed by vaccination with DCs (1 × 10⁵ cells) pulsed with WT1_236Y or MAGE-A4_143-151 as Figure 6. On day 16, PBMCs and tumor tissues were harvested and subjected to FACS (A) and immunohistochemical (B) analysis, respectively. A representative result from 2 independent experiments is shown.