Vaccination with DCs pulsed with WT1236Y enhanced WT1-specific CAR-T cells to suppress growth of WT1-positive tumors. (A) Schematic representation for the adoptive transfer experiment using NOG mice. (B) Tumor growth curves of K562-A24 in NOG mice (n = 4) transferred with CAR-T cells together with or without DCs pulsed with a relevant or irrelevant peptide. NOG mice bearing 3-day-old K562-A24 tumors were transferred with #213 scFv CAR-T cells or CEA-specific (F11-39 scFv) CAR-T cells (1 × 107 cells) together with or without DCs (1 × 105 cells) pulsed with WT1236Y or MAGE-A4143-151. Tumor volumes were measured by a caliper using the formula (length × width) at the indicated time points. Error bars represent SD of the mean. *P < .05; **P < .01. A representative result from 2 independent experiments is shown.