Adoptive transfer of WT1-specific CAR-T cells suppressed tumor growth in an antigen-specific manner. (A) Schematic representation for the adoptive transfer experiment using NOG mice. (B) Tumor growth curves of K562 and K562-A24 in NOG mice (n = 4) transferred with CAR-T cells or mock transduced T cells. NOG mice were inoculated s.c. with K562 and K562-A24 (5 × 10⁶ cells) followed by IV injection with #213 CAR-T cells or mock transduced T cells (1 × 10⁷ cells). Tumor volumes were measured by a caliper using the formula (length × width) at the indicated time points (n = 5). Error bars represent SD of the mean. *P < .05. A representative result from 2 independent experiments is shown. s.c., subcutaneous; TBI, total body irradiation.