Desialylated GPIbα^−/−platelets cannot rescue hepatic TPO generation in vivo. Representative line graphs of baseline desialylation levels of WT and GPIbα^−/− platelets (A) and desialylation of GPIbα^−/− platelets before and after neuraminidase treatment (B), as determined by fluorescein-conjugated RCA-1 binding. (C) WT mice were transfused with 2.5 × 10⁸ desialylated or control GPIbα^−/− platelets. Livers were harvested 24 hours posttransfusion, and TPO mRNA was quantified by RT-qPCR. n = 3. NS, not significant.