Analysis for the presence of proT1-derived and proT2-derived cells in the thymus of competitively reconstituted immunodeficient mice. (A) 1:1 mixture of sorted HLA-A2⁻ proT1-cells (1 × 10⁵) and sorted HLA-A2⁺ (1 × 10⁵) proT2-cells were injected into nonirradiated NSG neonatal mice and the thymuses harvested and analyzed after 17 days (n = 7 mice/group). Shown is flow cytometric analysis of human CD45 and HLA-A2 cell surface expression and CD5, CD7, CD8, and CD4 expression on CD45⁺HLA-A2⁻– and CD45⁺HLA-A2⁺–gated cells for proT1- or proT2-derived cells, respectively. (B) Percentage of HLA-A2⁻ or HLA-A2⁺ cells over total human CD45⁺ cells for individual mice shown. The results shown are representative of at least 3 independent experiments. *P < .05.