Continuous AhR activation is permissive to erythroid cell maturation. (A) Representative FACS analysis of cells coexpressing 2 hallmark markers of the erythroid lineage: CD71 (transferrin receptor, early) and CD235 (glycophorin a, late) on day 15 and day 30 of specification. (B) Representative FACS analysis of cells coexpressing CD235E and CD41-demonstrating that by day 60, virtually all of the cells are committed to the erythroid lineage. (C) Wright-Giemsa staining of day 30 erythroid-lineage cells pre- and postexposure to hypoxic conditions demonstrating decreased size and condensed chromatin under low oxygen conditions. (D) Hemoglobin-expressing cell pellet of iPSC-derived erythroid-lineage cells.