DOCK2 is recruited to the synapse in a manner dependent on the PI3K activity. NK cells from DOCK2-GFP mice were treated with wortmannin (100 nM), LY294002 (10 μM), FIPI (750 nM), R59-022 (10 μM), or Mock (DMSO) for 30 minutes at 37°C. Cells were then mixed with YAC-1 cells for 5 minutes to analyze localization of DOCK2 (GFP) and F-actin in the conjugates. Scale bar, 5 μm. Data are expressed as the percentages of the conjugates with polarized accumulation of F-actin or DOCK2 (the mean ± SD) of 3 independent experiments. In each experiment, more than 30 cells were analyzed per group. **P < .01.