NK cell–mediated cytotoxicity is impaired in the absence of DOCK2. (A) WT and DOCK2^−/− NK cells were incubated with CHO cells at the indicated ratio in the presence or absence of anti-Ly49D blocking antibody (10 μg/mL). (B) WT and DOCK2^−/− NK cells were incubated with EL4 cells with or without pretreatment with anti-Thy1.2 antibody (10 μg/mL) at the indicated ratio. (C) Fresh (left) or in vitro-activated (right) WT and DOCK2^−/− NK cells were incubated with EL-4 or EL4-Rae1ε cells at the indicated ratio. (D) Fresh (left) or in vitro-activated (right) WT and DOCK2^−/− NK cells were incubated with YAC-1 cells at the indicated ratio. (E) In vitro–activated WT and DOCK2^−/− NK cells were incubated with YAC-1 cells at the ratio of 1:1 or cultured alone in the presence of IL-12 to measure IFN-γ production. In (A–E), data are expressed as the mean ± SD of triplicate wells and are representative of at least 2 independent experiments. *P < .05; **P < .01.