Figure 5
Figure 5. Immunohistochemistry of PTEN and pAKT performed in SS and MF skin biopsies. (A) Hut78 as a positive control for PTEN (original magnification ×40/0.7 numerical aperture); (B-C) PTEN immunoreactivity in SS skin (B, original magnification ×20/0.6 numerical aperture; C, original magnification ×10/0.3 numerical aperture); (D) PTEN immunoreactivity in MF (original magnification ×20/0.6 numerical aperture); (E) HH as a negative control for PTEN (original magnification ×40/0.7 numerical aperture); (F-G) pAKT immunostaining associated with the cytoplasm of keratinocytes and scattered malignant lymphocytes (original magnification ×20/0.6 numerical aperture); and (H) strong pAKT positivity associated with the cytoplasm of SS cells and particularly with actively dividing mitotic cells (original magnification ×40/0.7 numerical aperture). Digital images were acquired with an AxioCam MRc5 camera (Zeiss) and an Axioskop2 microscope (Zeiss).

Immunohistochemistry of PTEN and pAKT performed in SS and MF skin biopsies. (A) Hut78 as a positive control for PTEN (original magnification ×40/0.7 numerical aperture); (B-C) PTEN immunoreactivity in SS skin (B, original magnification ×20/0.6 numerical aperture; C, original magnification ×10/0.3 numerical aperture); (D) PTEN immunoreactivity in MF (original magnification ×20/0.6 numerical aperture); (E) HH as a negative control for PTEN (original magnification ×40/0.7 numerical aperture); (F-G) pAKT immunostaining associated with the cytoplasm of keratinocytes and scattered malignant lymphocytes (original magnification ×20/0.6 numerical aperture); and (H) strong pAKT positivity associated with the cytoplasm of SS cells and particularly with actively dividing mitotic cells (original magnification ×40/0.7 numerical aperture). Digital images were acquired with an AxioCam MRc5 camera (Zeiss) and an Axioskop2 microscope (Zeiss).

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