American Society of Hematology

Figure 2

$Figure 2. PTEN levels measured in SS patients. (A) qRT-PCR analysis of PTEN performed in 17 SS patients. The PTEN mRNA level was calculated for each SS patient relatively to a reference pool consisting of 5 healthy controls (black bar). The bottom portion indicates the percentage of clonal TCR vβ+ cells within the CD4+ fraction and the PTEN status represented as wild type (+/+), heterozygous deletion (+/−), and homozygous deletion (−/−). Samples were measured in 2 independent experiments performed in triplicate. (B) Western blotting analysis of PTEN in the purified CD4+ cells of 19 SS individuals grouped by PTEN +/+, +/−, and −/−, and UPD genomic status. Two representative HDs of the 5 analyzed are shown in the figure. Because of the timing of patient recruitment, the gels were run at separate times and the lanes were composed for the figure.$

PTEN levels measured in SS patients. (A) qRT-PCR analysis of PTEN performed in 17 SS patients. The PTEN mRNA level was calculated for each SS patient relatively to a reference pool consisting of 5 healthy controls (black bar). The bottom portion indicates the percentage of clonal TCR vβ⁺ cells within the CD4⁺ fraction and the PTEN status represented as wild type (+/+), heterozygous deletion (+/−), and homozygous deletion (−/−). Samples were measured in 2 independent experiments performed in triplicate. (B) Western blotting analysis of PTEN in the purified CD4⁺ cells of 19 SS individuals grouped by PTEN +/+, +/−, and −/−, and UPD genomic status. Two representative HDs of the 5 analyzed are shown in the figure. Because of the timing of patient recruitment, the gels were run at separate times and the lanes were composed for the figure.

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