Inactivation of Bim minimally rescues the expansion of alloantigen-activated Ezh2-deficient T cells in vivo. (A-C) Naïve CD4⁺ T cells from WT or T-KO B6 mice were stimulated with CD3/CD28 Abs. Three days later, total RNA and protein lysates were prepared. Western blot analysis shows the amount of histone methylation marks (A). The graph shows the gene expression analyzed by RT-PCR (B). Western blot analysis shows the protein levels (C). Data are representative of 2 independent experiments. (D) Donor T cells (CD4⁺ + CD8⁺ T cells) derived from WT, Ezh2-T-KO, Bim^−/−, or Bim^−/− EZH2-T-KO mice were transplanted with TCD BM into lethally irradiated BALB/C recipients. Seven days later, cells were recovered from these recipients, counted, and analyzed using flow cytometry. Data are representative of 2 independent experiments, each with 3 to 4 recipients per group. Dot plots show the fraction of donor-derived CD4⁺ T cells. Graphs show the absolute number of donor CD4⁺ cells. (E) WT, Ezh2-T-KO, Bim^−/−, or Bim^−/− Ezh2-T-KO B6 mouse-derived T cells were cultured with BALB/C BM-derived DCs. Graphs show the recovery rate of live cells. *P < .05, **P < .001, ***P < .001. Error bars indicate mean ± SD. Data are representative of 2 independent experiments.