Safety of T cells expressing enhanced affinity WT1-specific TCRs in mice infected with LM_WT1. (A) TCR-transduced P14 T cells were transferred into B6 mice and, after 18 days, challenged with LM or LM_WT1. After 6 days, blood from treated mice was analyzed by flow cytometry. Data are representative of 3 independent experiments with at least 1 LM and 2 LM_WT1 mice per experiment. (B) BM cells were purified from treated mice, and the percentage of CD117⁺ Sca-1^int and Sca-1^hi cells was determined. Data shown are from an experiment in which a single mouse per TCR construct was immunized with control LM and 2 mice were challenged with recombinant LM_WT1. (C) Kidney and lung were also collected from these animals and analyzed by hematoxylin and eosin staining for T-cell infiltration and/or tissue damage.