DNA methylation and gene expression of the CD8A gene as representative rHMR. Detailed illustration of CD8A as an example of a potentially rHMR. (A) Gene expression (ie, messenger RNA) levels of CD8A in leukocyte cell subsets. Gene expression is given as log-fold change of signal intensity. (B) DNA methylation profile of the CD8A gene in individual cell subsets. (Top panel) HMRs identified by the K450 array in the different cell subsets are displayed as a colored bar indicating extend of the HMR. (Middle panel) Raw MeDIP-seq analyses are plotted as number of mapped methylated DNA fragments. The extent of HMRs within CD8⁺ T-cells is indicated by a black bar. (Bottom panel) The location of CGIs (blue boxes), location of K450 array probes, and the position of the CD8A gene mapped to the reference genome. TSS and gene orientation are indicated by a black arrow.