Fev regulation of HSC development is cell autonomous. (A) Transplantation scheme: rhodamine-labeled donor cells at the ventral marginal zone of cmyb:GFP transgenic donor embryos were transplanted into wild-type recipients at the blastula stage. (B) HSC reconstitution in the CHT of recipient embryos at 36 hpf. Green, cmyb:GFP⁺ cells; red, rhodamine. White arrows, GFP⁺ HSCs contributed by donor cells; light blue arrowheads, donor-derived GFP⁻ hematopoietic cells. (C) Summary of transplantation results. Note that only host embryos with donor-derived rhodamine⁺ hematopoietic cells in the CHT were included here.