VEGF-A:VEGFR2 complexes and total VEGF-D in inflamed footpads are decreased when neutrophil accumulation is attenuated. (A) Footpad skin whole mounts were examined for lymphatic vessels in mice treated with anti-VEGFR2, anti-VEGFR3, or control rat IgG. (B) Density of lymphatic vessels in footpads from immunized mice treated with anti-VEGFR2, anti-VEGFR3, or control rat IgG. (C) VEGF-A ELISA of footpad homogenates from NIMP-R14- and control rat IgG-treated mice at various times after immunization. (D) Footpad sections were immunostained for VEGF-A:VEGFR2 complexes in NIMP-R14- and control rat IgG-treated mice. (E) VEGF-D ELISA of footpad homogenates from NIMP-R14- and control rat IgG-treated mice at various times postimmunization. (F) VEGF-D ELISA of supernatant harvested from nonstimulated and fMLP-stimulated neutrophils. Data from panels A and B are pooled from or representative of 2 independent experiments, with 3 mice per group in each experiment (n = 6). Scale bars in panel A represent 75 μm. Bars in panel B represent mean ± SD. *P < .05; **P < .01. Images from panel D are representative of 4 independent experiments (n = 4). Scale bars in panel D represent 50 μm. ELISA data from panels C and E are pooled from 2 independent experiments with 2 to 3 mice per group in each experiment (n = 5-6) and represent mean ± SD. *P < .05; **P < .01; NS, not significant. ELISA data from panel F are derived from neutrophils isolated from 4 different mice (n = 4).