Figure 2
CD40L+ CD8+ T cells in human antigen–specific responses. (A) Human PBMCs from healthy donors were stimulated with peptide pools from different virus antigens. The dot-plots show representative intracellular cytokine and CD40L staining of CD3+ CD8+ CD4−–gated lymphocytes. (B) The frequencies of CD40L+ and IFN-γ+ virus-specific CD8+ T cells and the corresponding ratio of CD40L+ cells among virus-specific IFN-γ+ CD8+ T cells are summarized for CMV (n = 16), EBV (n = 17), and flu (n = 26) (mean ± SEM). (C) At different time points after YFV vaccination, PBMCs from healthy donors were stimulated with the immunodominant peptide YFV-NS4B. The dot-plots show the intracellular IFN-γ and CD40L staining of CD3+ CD8+ CD4−–gated lymphocytes from 1 representative donor (n = 6).

CD40L+ CD8+ T cells in human antigen–specific responses. (A) Human PBMCs from healthy donors were stimulated with peptide pools from different virus antigens. The dot-plots show representative intracellular cytokine and CD40L staining of CD3+ CD8+ CD4–gated lymphocytes. (B) The frequencies of CD40L+ and IFN-γ+ virus-specific CD8+ T cells and the corresponding ratio of CD40L+ cells among virus-specific IFN-γ+ CD8+ T cells are summarized for CMV (n = 16), EBV (n = 17), and flu (n = 26) (mean ± SEM). (C) At different time points after YFV vaccination, PBMCs from healthy donors were stimulated with the immunodominant peptide YFV-NS4B. The dot-plots show the intracellular IFN-γ and CD40L staining of CD3+ CD8+ CD4gated lymphocytes from 1 representative donor (n = 6).

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