Activation of the NLRP3 inflammasome in platelets from DV-infected patients. (A-B) Representative density plots showing the expression of NLRP3 in (A) platelets and (B) MPs from healthy subjects (control) or patients with dengue (dengue). The values in each region of the quadrants indicate the cell frequencies in the respective quadrant. (C) Platelets obtained from healthy subjects and patients with dengue were stained with anti-NLRP3 (green) and anti-ASC (red) and examined by confocal microscopy using an Olympus FV10i-O with a 240× lens. The images were processed using FLUOVIEW FV1000 MPE software version 5.0 and further analyzed using Adobe Photoshop CS Version 8.0. Bars represent 10 µm. (D) Caspase-1 activation in platelets isolated from control participants and dengue patients was assessed by the MFI of the fluorescent probe FLICA. The boxes indicate the median and interquartile ranges and the whiskers indicate 5 to 95 percentile. **P < .01 compared with healthy volunteers. (E) Western blot analysis of pro (p31) and cleaved (p17) IL-1β and of β-actin in platelets that were isolated from 3 healthy volunteers and 3 dengue patients.