Figure 4
Figure 4. Human GALT reconstitution in TKO-BLT mice. (A) Representative sections of 22 wpt small and large intestine from DKO-BLT (n = 3) and TKO-BLT (n = 3) mice were stained for the presence of human cells (HLA class I+) and for HIV-susceptible CD4+ cells. Magnified ×200; numerical aperture, 0.75; Olympus DP72 camera and BX51 microscope. Acquisition software: Olympus cell Sens Dimension 1.4.1. (B) Flow cytometry plots showing cellular subsets and T-cell activation status in GALT from a TKO-BLT mouse at 19 wpt (left to right: CD3+ T cells; CD4+/CD8+ T cells; Tcm [CD45RA−CCR7+] and Tem [CD45RA−CCR7−] subsets; activated HLA-DR+ T cells; CD11c+ myeloid and CD123+ plasmacytoid DCs). Parent gates are listed above each panel. Gating was based on distinct populations in mesenteric lymph nodes that were run on the same day.

Human GALT reconstitution in TKO-BLT mice. (A) Representative sections of 22 wpt small and large intestine from DKO-BLT (n = 3) and TKO-BLT (n = 3) mice were stained for the presence of human cells (HLA class I+) and for HIV-susceptible CD4+ cells. Magnified ×200; numerical aperture, 0.75; Olympus DP72 camera and BX51 microscope. Acquisition software: Olympus cell Sens Dimension 1.4.1. (B) Flow cytometry plots showing cellular subsets and T-cell activation status in GALT from a TKO-BLT mouse at 19 wpt (left to right: CD3+ T cells; CD4+/CD8+ T cells; Tcm [CD45RA−CCR7+] and Tem [CD45RA−CCR7−] subsets; activated HLA-DR+ T cells; CD11c+ myeloid and CD123+ plasmacytoid DCs). Parent gates are listed above each panel. Gating was based on distinct populations in mesenteric lymph nodes that were run on the same day.

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