Peripheral tissue reconstitution and splenic structure in TKO-BLT mice. (A) Representative flow cytometry plots with means ± standard deviations illustrate the major human immune cell subset frequencies (from left to right: CD4⁺ and CD8⁺ T cells; CD3⁺ T cells and CD19⁺ B cells; CD33⁺ myeloid cells and CD14⁺ monocytes; CD11c⁺ myeloid DC and CD123⁺ plasmacytoid DCs) in bone marrow (n = 6), mesenteric lymph node (n = 4), and spleen (n = 6) and (B) the splenic T-cell subsets (n = 6) (naïve CD45RA⁺CCR7⁺; T_cm CD45RA⁻CCR7⁺; T_em CD45RA⁻CCR7⁻; T_EMRA CD45RA⁺CCR7⁻) in TKO-BLT mice. Parental gates are listed at the top of each column. (C) Histological staining depicting the characteristic morphology of TKO-BLT spleens, the presence of HLA class I–expressing human cells, and follicle-like structures containing mature CD20⁺ B cells and CD3⁺ T cells. Olympus DP72 camera and BX51 microscope. Hematoxylin and eosin ×40, ×200, and immunostaining ×200 magnification; numerical aperture, 0.75. Acquisition software: Olympus cell Sens Dimension 1.4.1.